The Men's 1km time trial event at the 2010 South American Games was held on March 21.
| Gold | Silver | Bronze |
| Fabian Hernando Zapata Colombia |
Jonathan Cermeno Colombia |
Davi Romeo Brazil |
| Rank | Rider | 250m | 500m | 750m | Time |
|---|---|---|---|---|---|
| Fabian Hernando Zapata (COL) | 19.242 (3) | 32.851 (1) | 47.605 (1) | 1:03.953 | |
| Jonathan Cermeno (COL) | 19.257 (4) | 33.198 (3) | 48.118 (2) | 1:04.756 | |
| Davi Romeo (BRA) | 19.239 (2) | 33.838 (5) | 49.311 (5) | 1:05.656 | |
| 4 | Emiliano Fernandez (ARG) | 18.683 (1) | 33.034 (2) | 48.716 (3) | 1:05.787 |
| 5 | Darwin Villa (VEN) | 19.369 (5) | 33.548 (4) | 48.937 (4) | 1:06.041 |
| 6 | Fernando Fermino (BRA) | 20.415 (8) | 35.084 (8) | 50.459 (7) | 1:06.792 |
| 7 | Christopher Almonacid (CHI) | 19.689 (7) | 35.026 (7) | 50.643 (8) | 1:06.975 |
| 8 | Enzo Bruno Farias (CHI) | 19.660 (6) | 34.519 (6) | 50.402 (6) | 1:07.389 |
| 9 | Gabriel Aguiar (URU) | 21.595 (9) | 37.935 (9) | 55.141 (9) | 1:13.123 |
| Edgard Nicolas Palma (URU) | DNS |